Estudo de diferentes meios de cultivo celular para expressão de glicoproteína recombinante humana, em células de ovário de hamster chinês (cho)
Keywords:
Cultivo celular, CHO, Meio de cultivo, TSH, GlicoproteínasAbstract
Um cultivo de células eucariota refere-se à manutenção de células removidas a partir de tecido animal ou órgãos, as quais continuam a crescer com adição de fatores de crescimento e nutrientes. As células CHO (Células de Ovário de Hamster Chinês) são muito populares quanto à expressão de glicoproteína recombinante humana. Estas células possuem enzimas de glicosilação que se assemelham àquelas encontradas em linhagens de células humanas. A CHO é uma linhagem aderente passível de adaptação ao crescimento em suspensão e requer meio de cultivo líquido quimicamente complexo e adequado para o seu crescimento. No presente estudo, foi realizado um comparativo de quatro meios de cultivo para células CHO e a expressão da glicoproteína recombinante TSH (Hormônio Estimulante da Tireóide) em dois processos de cultivo, foi analisada.
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